{"id":5333,"date":"2017-05-31T11:47:10","date_gmt":"2017-05-31T09:47:10","guid":{"rendered":"https:\/\/www.famelab.gr\/?p=5333"},"modified":"2025-10-30T08:20:14","modified_gmt":"2025-10-30T08:20:14","slug":"low-protein-high-carbohydrate-diet-increases-browning-in-perirenal-adipose-tissue-but-not-in-inguinal-adipose-tissue","status":"publish","type":"post","link":"https:\/\/famelab.gr\/el\/low-protein-high-carbohydrate-diet-increases-browning-in-perirenal-adipose-tissue-but-not-in-inguinal-adipose-tissue\/","title":{"rendered":"Low-protein, high-carbohydrate diet increases browning in perirenal adipose tissue but not in inguinal adipose tissue"},"content":{"rendered":"

\"FAME<\/a>Pereira MP, Ferreira LAA, da Silva FHS, Christoffolete MA, Metsios GS, Chaves VE, de Fran\u00e7a SA, Damazo AS, Flouris AD<\/a>, Kawashita NH. A low-protein, high-carbohydrate diet increases browning in perirenal adipose tissue but not in inguinal adipose tissue. Nutrition. 2017 Oct;42:37-45. doi: 10.1016\/j.nut.2017.05.007. Epub 2017 May 31. PMID: 28870477.<\/span><\/p>\n

Abstract:<\/strong><\/span><\/p>\n

Objective: The aim of this study was to evaluate the browning and origin of fatty acids (FAs) in the maintenance of triacylglycerol (TG) storage and\/or as fuel for thermogenesis in perirenal adipose tissue (periWAT) and inguinal adipose tissue (ingWAT) of rats fed a low-protein, high-carbohydrate (LPHC) diet.<\/p>\n

Methods: LPHC (6% protein, 74% carbohydrate) or control (C; 17% protein, 63% carbohydrate) diets were administered to rats for 15 d. The tissues were stained with hematoxylin and eosin for histologic analysis. The content of uncoupling protein 1 (UCP1) was determined by immunofluorescence. Levels of T-box transcription factor (TBX1), PR domain containing 16 (PRDM16), adipose triacylglycerol lipase (ATGL), hormone-sensitive lipase, lipoprotein lipase (LPL), glycerokinase, phosphoenolpyruvate carboxykinase (PEPCK), glucose transporter 4, \u03b23-adrenergic receptor (AR), \u03b21-AR, protein kinase A (PKA), adenosine-monophosphate-activated protein kinase (AMPK), and phospho-AMPK were determined by immunoblotting. Serum fibroblast growth factor 21 (FGF21) was measured using a commercial kit (Student\u2019s t tests, P < 0.05).<\/p>\n

Results: The LPHC diet increased FGF21 levels by 150-fold. The presence of multilocular adipocytes, combined with the increased contents of UCP1, TBX1, and PRDM16 in periWAT of LPHC-fed rats, suggested the occurrence of browning. The contents of \u03b21-AR and LPL were increased in the periWAT. The ingWAT showed higher ATGL and PEPCK levels, phospho-AMPK\/AMPK ratio, and reduced \u03b23-AR and PKA levels.<\/p>\n

Conclusion: These findings suggest that browning occurred only in the periWAT and that higher utilization of FAs from blood lipoproteins acted as fuel for thermogenesis. Increased glycerol 3-phosphate generation by glyceroneogenesis increased FAs reesterification from lipolysis, explaining the increased TG storage in the ingWAT.<\/p>\n

Full Text Link: <\/strong><\/span><\/p>\n

https:\/\/pubmed.ncbi.nlm.nih.gov\/28870477\/<\/a><\/p>\n

\"\"<\/p>\n

 <\/p>\n

 <\/p>","protected":false},"excerpt":{"rendered":"

Pereira MP, Ferreira LAA, da Silva FHS, Christoffolete MA, Metsios GS, Chaves VE, de Fran\u00e7a SA, Damazo AS, Flouris AD, Kawashita NH. A low-protein, high-carbohydrate diet increases browning in perirenal adipose tissue but not in inguinal adipose tissue. Nutrition. 2017 Oct;42:37-45. doi: 10.1016\/j.nut.2017.05.007. Epub 2017 May 31. PMID: 28870477. Abstract: Objective: The aim of this […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[42],"tags":[],"class_list":["post-5333","post","type-post","status-publish","format-standard","hentry","category-fame-lab-publications-2017"],"_links":{"self":[{"href":"https:\/\/famelab.gr\/el\/wp-json\/wp\/v2\/posts\/5333","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/famelab.gr\/el\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/famelab.gr\/el\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/famelab.gr\/el\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/famelab.gr\/el\/wp-json\/wp\/v2\/comments?post=5333"}],"version-history":[{"count":1,"href":"https:\/\/famelab.gr\/el\/wp-json\/wp\/v2\/posts\/5333\/revisions"}],"predecessor-version":[{"id":16602,"href":"https:\/\/famelab.gr\/el\/wp-json\/wp\/v2\/posts\/5333\/revisions\/16602"}],"wp:attachment":[{"href":"https:\/\/famelab.gr\/el\/wp-json\/wp\/v2\/media?parent=5333"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/famelab.gr\/el\/wp-json\/wp\/v2\/categories?post=5333"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/famelab.gr\/el\/wp-json\/wp\/v2\/tags?post=5333"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}