{"id":9333,"date":"2022-04-10T11:26:21","date_gmt":"2022-04-10T09:26:21","guid":{"rendered":"https:\/\/www.famelab.gr\/?p=9333"},"modified":"2025-10-30T07:49:22","modified_gmt":"2025-10-30T07:49:22","slug":"irisin-regulates-thermogenesis-and-lipolysis-in-3t3-l1-adipocytes","status":"publish","type":"post","link":"https:\/\/famelab.gr\/el\/irisin-regulates-thermogenesis-and-lipolysis-in-3t3-l1-adipocytes\/","title":{"rendered":"Irisin regulates thermogenesis and lipolysis in 3T3-L1 adipocytes"},"content":{"rendered":"<p><span style=\"color: #000000;\"> <a href=\"https:\/\/famelab.gr\/el\/irisin-regulates-thermogenesis-and-lipolysis-in-3t3-l1-adipocytes\/\"><img fetchpriority=\"high\" decoding=\"async\" class=\"alignright wp-image-9334 size-full\" title=\"Irisin regulates thermogenesis and lipolysis in 3T3-L1 adipocytes - FAME Lab\" src=\"https:\/\/www.famelab.gr\/wp-content\/uploads\/2022\/04\/FAME-Lab-Irisin-regulates-thermogenesis-and-lipolysis-in-3T3-L1-adipocytes.jpg\" alt=\"Irisin regulates thermogenesis and lipolysis in 3T3-L1 adipocytes - FAME Lab\" width=\"218\" height=\"244\" \/><\/a><a href=\"https:\/\/famelab.gr\/el\/people\/\" target=\"_blank\" rel=\"noopener noreferrer\">Vliora M<\/a>, Grillo E, Corsini M, Ravelli C, <a href=\"https:\/\/famelab.gr\/el\/people\/\" target=\"_blank\" rel=\"noopener noreferrer\">Nintou E<\/a>, <a href=\"https:\/\/famelab.gr\/el\/people\/\" target=\"_blank\" rel=\"noopener noreferrer\">Karligiotou E,<\/a> <a href=\"https:\/\/famelab.gr\/el\/people\/\" target=\"_blank\" rel=\"noopener noreferrer\">Flouris AD<\/a>, Mitola S. Irisin regulates thermogenesis and lipolysis in 3T3-L1 adipocytes. Biochim Biophys Acta Gen Subj. 2022 Apr;1866(4):130085. doi: 10.1016\/j.bbagen.2022.130085. Epub 2022 Jan 10. PMID: 35016977.<\/span><\/p>\n<p><span style=\"color: #000000;\"><strong>Abstract:<\/strong><\/span><\/p>\n<p>Background: Adipose tissue plays a pivotal role in the development and progression of the metabolic syndrome which along with its complications is an epidemic of the 21st century. Irisin is an adipo-myokine secreted mainly by skeletal muscle and targeting, among others, adipose tissue. In brown adipose tissue it upregulates uncoupling protein-1 (UCP1) which is responsible for mitochondrial non-shivering thermogenesis.<\/p>\n<p>Methods: Here we analyzed the effects of irisin on the metabolic activity of 3T3-L1 derived adipocytes through a mitochondrial flux assay. We also assessed the effects of irisin on the intracellular signaling through Western Blot. Finally, the gene expression of ucp1 and lipolytic genes was examined through RT-qPCR.<\/p>\n<p>Results: Irisin affects mitochondrial respiration and lipolysis in a time-dependent manner through the regulation of PI3K-AKT pathway. Irisin also induces the expression of UCP1 and the regulation of NF-\u03baB, and CREB and ERK pathways.\u00a0Conclusion: Our data supports the role of irisin in the induction of non-shivering thermogenesis, the regulation of energy expenditure and lipolysis in adipocytes. General significance: Irisin may be an attractive therapeutic target in the treatment of obesity and related metabolic disorders.<\/p>\n<p><span style=\"color: #000000;\"><strong>Full Text Link:<\/strong><\/span><\/p>\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/35016977\/\" target=\"_blank\" rel=\"noopener noreferrer\">https:\/\/pubmed.ncbi.nlm.nih.gov\/35016977\/<\/a><\/p>\n<p><span style=\"color: #000000;\"><strong>Download PDF:<\/strong><\/span><\/p>\n<p><a href=\"https:\/\/www.famelab.gr\/wp-content\/uploads\/2022\/05\/Irisin-regulates-thermogenesis-and-lipolysis-in-3T3-L1-adipocytes_2022_Main-text_Irisin_R1.pdf\" target=\"_blank\" rel=\"noopener noreferrer\">https:\/\/www.famelab.gr\/wp-content\/uploads\/2022\/05\/Irisin-regulates-thermogenesis-and-lipolysis-in-3T3-L1-adipocytes_2022_Main-text_Irisin_R1.pdf<\/a><\/p>\n<p><img decoding=\"async\" class=\"alignnone wp-image-8302 size-full\" src=\"https:\/\/www.famelab.gr\/wp-content\/uploads\/2021\/11\/Line.png\" alt=\"\" width=\"1316\" height=\"1\" \/><\/p>\n<p>&nbsp;<\/p>","protected":false},"excerpt":{"rendered":"<p>Vliora M, Grillo E, Corsini M, Ravelli C, Nintou E, Karligiotou E, Flouris AD, Mitola S. Irisin regulates thermogenesis and lipolysis in 3T3-L1 adipocytes. Biochim Biophys Acta Gen Subj. 2022 Apr;1866(4):130085. doi: 10.1016\/j.bbagen.2022.130085. Epub 2022 Jan 10. PMID: 35016977. Abstract: Background: Adipose tissue plays a pivotal role in the development and progression of the metabolic [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11],"tags":[],"class_list":["post-9333","post","type-post","status-publish","format-standard","hentry","category-fame-lab-publications-2022"],"_links":{"self":[{"href":"https:\/\/famelab.gr\/el\/wp-json\/wp\/v2\/posts\/9333","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/famelab.gr\/el\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/famelab.gr\/el\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/famelab.gr\/el\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/famelab.gr\/el\/wp-json\/wp\/v2\/comments?post=9333"}],"version-history":[{"count":1,"href":"https:\/\/famelab.gr\/el\/wp-json\/wp\/v2\/posts\/9333\/revisions"}],"predecessor-version":[{"id":16541,"href":"https:\/\/famelab.gr\/el\/wp-json\/wp\/v2\/posts\/9333\/revisions\/16541"}],"wp:attachment":[{"href":"https:\/\/famelab.gr\/el\/wp-json\/wp\/v2\/media?parent=9333"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/famelab.gr\/el\/wp-json\/wp\/v2\/categories?post=9333"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/famelab.gr\/el\/wp-json\/wp\/v2\/tags?post=9333"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}